1861
| RANK | NAME |
| Colonel | Richard H. Rush |
| Lieutenant Colonel | John H. McArthur |
| Majors | C. Ross Smith |
| Robert Morris, Jr. | |
| Adjutant | Frederick C. Newhall |
| Quartermaster | Thomas E. Maley |
| Surgeons | William Moss |
| Charles M. Ellis | |
| Chaplain | Washington R. Erben |
| Captains | George E. Clymer |
| William P. C. Treichel | |
| John H. Gardiner | |
| Henry C. Whalen | |
| Joseph Wright | |
| J. Henry Hazeltine | |
| Robert Milligan | |
| Benoni Lockwood | |
| James Starr | |
| Howard Ellis | |
| 1st Lieutenants | Augustus Bertolette |
| R. Walsh Mitchell | |
| Charles L. Leiper | |
| Henry P. Muirhead | |
| Samuel Hazzard, Jr | |
| G. Irvine Whitehead | |
| Charles E. Richards | |
| Charles E. Cadwalader | |
| Oswald Jackson | |
| John W. Williams | |
| 2nd Lieutenants | William B. Call |
| William W. Frazier, Jr. | |
| Emlen N. Carpenter | |
| Charles B. Davis | |
| J. Newton Dickson | |
| J. Hinckley Clark | |
| Edwin L. Tevis | |
| William Odenheimer | |
| Frank Furness | |
| Thomas W. Neill | |
June 1863
| RANK | NAME |
| Colonel | Richard H. Rush |
| Lieutenant Colonel | C. Ross Smith |
| Majors | Robert Morris, Jr. |
| Henry C. Whalen | |
| J. Henry Hazeltine | |
| Adjutant | Rudulph Ellis |
| Quartermaster | Theodore Sage |
| Surgeons | John B. Coover |
| Thomas S. Morrison | |
| Chaplain | Samuel L. Gracey |
| Captains | Frederick C. Newhall |
| William P. C. Treichel | |
| Charles E. Cadwalader | |
| William W. Frazier, Jr. | |
| Charles L. Leiper | |
| Charles B. Davis | |
| J. Newton Dickson | |
| J. Hinckley Clark | |
| James Starr | |
| Henry Winsor, Jr. | |
| 1st Lieutenants | R. Walsh Mitchell |
| Frank Furness | |
| Thomas W. Neill | |
| Albert P. Morrow | |
| John Riddle | |
| Edward L. Tevis | |
| G. Irvine Whitehead | |
| Samuel Smith | |
| Eugene P. Bertrand | |
| John Hendricks | |
| Abraham D. Price | |
| 2nd Lieutenants | Osgood We;sh |
| William White | |
| Charles P. Coxe | |
| George G. Meade, Jr. | |
| Thompson Lennig | |
| Thomaas J. Gregg | |
| Samuel R. Colladay | |
| Richard M. Sheppard | |
| Archer Maris | |
| Edward Whiteford | |
| Phillip H. Ellis | |
| Bernard H. Herkness |
JUNE 1865
| RANK | NAME |
| Colonel | Charles L. Leiper |
| Lieutenant Colonel | Albert P. Morrow |
| Majors | Abraham D. Price |
| Charles B. Coxe | |
| Bernard H. Herkness | |
| Adjutant | Charles A. Newhall |
| Quartermaster | J. W. McIlhenny |
| Surgeons | Daniel B. Swift |
| William B. Henderson | |
| Joseph J. Yocum | |
| Chaplain | Samuel L. Gracey |
| Captains | Archer Maris |
| Edward Whiteford | |
| Richard M. Sheppard | |
| Charles A. Vernon | |
| Andrew L. Lanigan | |
| Issac T. Moffatt | |
| Samuel R. Colladay | |
| T. Campbell Oakman | |
| William R. Wright | |
| James H. Workman | |
| Edward J. Hazel | |
| 1st Lieutenants | Charles White |
| Joseph D. Price | |
| Henry J. Toudy | |
| Henry B. Hertz | |
| William Scott | |
| Michael J. Golden | |
| William Carey | |
| John M. Oldenheimer | |
| Abiah T. Smedley | |
| 2nd Lieutenants | George W. Buckingham |